Thomas Guiraud's resume, Research associate specializing in molecular biology

Molecular biology
- DNA/RNA extraction from bacterial, animal and plant matrixes.
- Genomic DNA/cDNA quantification with real-time PCR methods (SYBR® Green, TaqMan®, duplex) or with hybridization methods (Northern, Southern, in situ hybridization).
Genetic engineering
- Cloning of genes and promoters.
- Construction of plasmid vectors (restriction/ligation, PCR fusion or Gateway).
Plant biotechnology
- A. tumefasciens mediated plant tranformation (Tomato, Tobacco, A. thaliana).
- Phenotyping and genotyping.
Microbiology
- Manipulation and detection of plant viruses (ELISA, immuno-RT-PCR).
- Bacteriophage isolation from environmental samples.
- Culture, characterization and quantification of bacteria by qPCR (Gram^- or Gram⁺).
- Quantification of cell viability by EMA-qPCR (Ethidium MonoAzide).

Research experience

Since 2012

Postdoctoral fellowship in the "Feed and Food" Department of Bordeaux Sciences Agro at Gradignan (Gironde, France). Supervisor : Michel LE HÉNAFFMichel LE HÉNAFF [michel.lehenaff@agro-bordeaux.fr].
- Development of a Taqman duplex qPCR test for Flavobacterium psychrophilum quantification, a pathogenic bacterium infecting rainbow trout.
- Optimization of fish eggs disinfection techniques through an EMA-qPCR method for cell viability quantification.
- Development of specific methods for genomic DNA extraction and quantification of probiotic bacteria in trout fry intestines.
Works resulting in publications (published or in preparation)¹ and conferences². Students mentoring.

Collaborations with the Pyrénées and Landes county laboratory (LDPL, Mont-de-Marsan, France), the Fish Health Protection Group from Aquitaine (GDSAA, Mont-de-Marsan, France), some fish farms from Aquitaine and the probiotic manufacturers Lallemand (Blagnac, France) and Dietaxion-Cobiotex (Pessac, France).
2010 - 2011

Research assistant in the team "Fruit organogenesis and Endoreduplication" (UMR 1332) on the INRA campus from Villenave d'Ornon (Gironde, France). Supervisor : Michel HERNOULDMichel HERNOULD [hernould@bordeaux.inra.fr].
- Development of an early detection test for floral initiation in order to monitor the impact of nitrogen on strawberry production.
Collaborations with Marie-Noëlle Deméné from Invenio center in Douville (Dordogne, France), economic interest grouping for fruits and vegetables sectors in Aquitaine.
2008 - 2010

Postdoctoral fellowship in the team "Fruit organogenesis and Endoreduplication" (UMR 1332) on the INRA campus from Villenave d'Ornon. Supervisor : Michel HERNOULD.
- Exploitation of a patent (INRA) concerning some qualities (size, taste) of tomato fruit.
- Transformation and profiling (phenotyping, genotyping and biochemical profiling) of tomato plants overaccumulating potassium in fruits.
Technician mentoring. Poster³.

Collaboration with Isabelle GAILLARD and Hervé SENTENAC from the team "KaliPHruit" in the Biochemistry and Plant Molecular Physiology laboratoratory from Montpellier.
2005 - 2008

Research assistant in the team "Fruit organogenesis and Endoreduplication" (UMR 1332) on the INRA campus from Villenave d'Ornon. Supervisors : Michel HERNOULD and Pierre BALDET.
- Cloning and characterization, in Tobacco (Nicotiana tabacum) and its ancestries (N. sylvestris and N. tomentosiformis), of coding and promoting sequences from "Superman" gene's isoforms involved in flower development.
- Transformation and profiling of tomato plants overaccumulating vitamin E in fruits.
Publication in preparation. Poster⁴.

Collaboration with Michel MATRINGE from the Biosciences and Biotechnology Institute of Grenoble.
2001 - 2004

PhD student in the team "Plant virology" (UMR 1332) in the INRA campus from Villenave d'Ornon. Supervisor : Olivier LE GALLOlivier LE GALL [olegall@bordeaux.inra.fr].
- Identification of viral genes overcoming plant resistance to Lettuce mosaic virus (LMV).
- Development of a PCR-based detection test for the "Most" pathogenic strains of LMV.
PhD manuscript. Publications^{5, 6}, posters and conferences. Student monitoring.

2002

Training workshop in the Jean-François LALIBERTÉ's lab at the Armand Frappier Institute in Laval (Québec, Canada).

Improving in PCR-based mutagenesis (site-directed or random) methods.

2000 - 2001

Post-graduate training in the team "Plant virology" (UMR 1332) in the INRA campus from Villenave d'Ornon. Supervisor : Frédéric REVERS.

Screening Recombinant Inbred Lines (RIL) of Arabidopsis thaliana to find and characterize new resistance genes for plant virus infection.

Intership report. Publication⁷.

1999 - 2000

Internship in the team "Fruit organogenesis and Endoreduplication" (UMR 1332) on the INRA campus from Villenave d'Ornon. Supervisors : Armand MOURAS and Michel HERNOULD.

Initiation to molecular biology, plant biotechnology and histology.

Education

2015

MOOC « Initiation to statistics with R software », « Concepts and methods in epidemiology », « Bioinformatics: Genomes and Algorithms ».

2004
PhD in Biology and Medical Sciences : University of Bordeaux (Gironde, France).

Publications

2015
Evaluation of glutaraldehyde, chloramine-T, bronopol, Incimaxx Aquatic® and hydrogen peroxide as biocides against Flavobacterium psychrophilum for sanitization of rainbow trout eyed eggs. Journal of Aquaculture Research & Development. Alexandra A. GRASTEAU, Thomas Th. GUIRAUD, Patrick P. DANIEL, Ségolène S. CALVEZ, Valérie V. CHESNEAU, Michel M. LE HÉNAFF. [Link]
2009
Involvement of the cylindrical inclusion (CI) protein in the overcoming of an eIF4E-mediated resistance against Lettuce mosaic potyvirus. Molecular Plant Pathology. Anas A. ABDUL-RAZZAK, Thomas Th. GUIRAUD, Martine M. PEYPELUT, Jocelyne J. WALTER, Marie-Christine M.-Chr. HOUVENAGHEL, Thierry Th. CANDRESSE, Olivier O. LE GALL, Sylvie S. GERMAN-RETANA. [Link]
2004
Specific detection of Lettuce mosaic virus isolates belonging to the “Most” type. Journal of Virological Methods. Martine M. PEYPELUT, Renate R. KRAUSE-SAKATE, Thomas Th. GUIRAUD, Marcelo Agenor M. A. PAVAN, Thierry Th. CANDRESSE, Francisco Murilo F. M. ZERBINI, Olivier O. LE GALL. [Link]
2004
Les facteurs viraux restaurant la compatibilité entre un potyvirus et une plante dont l'eIF4E est non-permissif. PhD manuscript (University of Bordeaux). Thomas Th. GUIRAUD, Olivier O. LE GALL (Supervisor). [Link]
2003
Multiple resistance phenotypes to Lettuce mosaic virus among Arabidopsis thaliana accessions. Molecular Plant-Microbe Interactions. Frédéric Fr. REVERS, Thomas Th. GUIRAUD, Marie-Christine M.-Chr. HOUVENAGHEL, Thierry Th. MAUDUIT, Olivier O. LE GALL, Thierry Th. CANDRESSE. [Link]

Other communications

2014
Nouvelle approche qPCR dans la détection de l’infection des truites arc-en-ciel par Flavobacterium psychrophilum. (Oral communication) 4^èmes Journées Recherche Filière Piscicole, Paris (France). Alexandra GRASTEAU, Thomas GUIRAUD, Valérie CHESNEAU, Patrick DANIEL, Michel LE HÉNAFF.
2010
Qualifruit - Breeding strategy for fruit size and organoleptic quality of tomato fruit. (Poster) Colloque Bilan des programmes ANR RIB 2006 et Émergence 2007, Marseille (France). Thomas GUIRAUD, Isabelle GAILLARD, Christophe ROTHAN, Christian CHEVALIER, Hervé SENTENAC, Michel HERNOULD.
2009
Modifying vitamin E in tomato fruit. (Poster) "Tomato genomics : new tools for improvement of yield and quality", EUSOL Workshop, Tolède (Espagne). Thomas GUIRAUD, Michel MATRINGE, Michel HERNOULD, Christophe ROTHAN, Pierre BALDET.

Research associate - Molecular biology

Main skills

Molecular biology

Genetic engineering

Plant biotechnology

Microbiology

Bioinformatics

Team and project management

Computer skills

Research experience

Education

Publications

Other communications

Hobbies and other interests